數位化病理組織分析平台
Digital Pathology Core Facility
P7-組織切片掃描及病理分析系統 -穿透光
TissueGnostics/ Transmission light
TissueGnostics/ Transmission light
儀器描述
TissueGnostics TissueFAXS SL 自動進片組織掃片設施,可針對免疫化學染色或螢光染色影像掃描後,進行細胞標誌染色個別拆解和定量,可呈現多目標與多樣性分析,分析過程可在數據和影像之間雙向追蹤,得知特定細胞群的形態和所在位置,最終可將所有數據輸出,匯入Excel或其他軟體進行統計分析。
主要特點
- 具備高解析視野拼接演算法,提供無縫拼接數位影像,及全景影像大圖輸出功能
- 連續景深堆疊演算法,降低穿透光及螢光背景干擾,提供樣本清晰影像
- 拍攝軟體具備整合式3D共軛焦掃描模組、景深延伸功能,可自由設定Z軸間距以及連續影像範圍
儀器規格
- 電動正立型顯微鏡:Zeiss Axio Imager 2
- Wildfield光源:Lumencor SpectraX 多波長LED燈源
- 螢光濾片:DAPI, FITC, TRITC, Cy5 (可支援Cy7的瑩光,但不可同時使用Cy5)
- 物鏡:2.5x, 10x, 20x, 63x油鏡
- 共軛焦掃描器:CrestOptics X-Light V2,70 um pinhole disk,5螢光通道:DAPI、FITC、TRITC、Cy5、Cy7
- 相機:
螢光Hamamatsu ORCA Flash4.0 sCMOS 2048 x 2048
穿透光Color CMOS 4.2Megapixel - 控制軟體:TISSUEGENOSTICS
- 離線分析軟體:StrataQuest PLUS
使用注意事項
- 玻片封片必須平整與乾淨,建議使用24 x 50 mm蓋玻片。
- 提供論片計價代掃服務,請洽技術專員
- 有委託掃片需求者請直接與管理員聯繫!
放置地點
國家生技研究園區A棟5樓A502c室
管理人員
技術專員:蕭志強
電子信箱:as0210241@gate.sinica.edu.tw
電話:02-7750-5500#1478
Description
TissueFAXS SL systems provide the benefits of both high-throughput of slides and laser-free fast confocal imaging. The Slide Validator technology to ensure a high level of digital slide sharpness. Each scanned FOV is evaluated for sharpness on-the-fly during the scan process.
Application
- Multi-channel fluorescence & brightfield
- Extended Focus Imaging & Z-Stacking
Specification
- Microscope main body:Zeiss Axio Imager 2
- Light source:Lumencor SPECTRA X Light engine LED
- Filter:DAPI, FITC, TRITC, Cy5 (support Cy7, but avoid use Cy5 at the same time)
- Objective:2.5x, 10x, 20x, 63x oil
- Confocal scanning head:CrestOptics X-Light V2,70 um pinhole disk,5 fluorescence channel:DAPI、FITC、TRITC、Cy5、Cy7
- Camera:Fluorescence:
Hamamatsu ORCA Flash4.0 sCMOS 2048x2048
Brightfield: Color CMOS 4.2Megapixel - Controller sofeware:TISSUEGENOSTICS
- OFF-Line sofeware:StrataQuest PLUS
Precautions for user
- The edge of slice sample must be smooth and clean
- Recommend to use 24 x 50 mm (0.17 mm)” coverslip
Facility location
National Biotechnology Research Park Building A, 5th Floor, room A502c
Manager
Contact:蕭志強
Enail:as0210241@gate.sinica.edu.tw
Tel:02-7750-5500#1478